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Daniel Wiegand, MSChE

Daniel is Co-founder, CEO and Board Director of EnPlusOne Bio. Previously, Daniel was an Advanced Technology Team staff member at the Wyss Institute for Biologically Inspired Engineering and Dr. George Church’s lab at Harvard Medical School. At the Wyss, Daniel was extensively involved in developing translational technology revolving around microarray based high throughput DNA/RNA oligonucleotide library synthesis, large scale gene assembly and fluorescence in situ gene sequencing (FISSEQ).

During his tenure at the Wyss Institute, Daniel lead the development of several cell free protein expression systems from non-standard bacteria such as Vibrio natriegens, Streptomyces coelicolor and Pseudomonas aeruginosa. His passion for protein engineering, mutagenesis and high-throughput expression techniques led to the development of novel enzymes that are currently being used to build a next-generation RNA oligonucleotide synthesis & manufacturing platform at EnPlusOne Bio. Daniel received his Bachelor of Science in Biochemistry and Master of Science in Chemical Engineering from Northeastern University.

Jonathan Rittichier, PhD

Jonathan is Co-founder and CSO of EnPlusOne Bio. He performed his graduate work in organic synthesis at Indiana University in Bloomington, Indiana. During his time there, he secured a Briscoe Fellowship and worked in the laboratory of Professor Michael Vannieuwenhze. Much of this work was toward synthesizing 1) novel fluorescent probes to spy on bacteria and 2) macrocyclic natural products to kill them. He joined George Church’s Research Group at Harvard Medical School where he helped develop an enzym